Cristiano Ronaldo's hopes of finishing as Europe's top scorer were dealt a blow as he failed to score as champions Juventus were beaten by Cagliari.
Ronaldo has scored an incredible 31 Serie A goals this season but found himself frustrated on Wednesday night.
Luca Gagliano's close-range finish and Giovanni Simeone's powerful drive secured the win for the hosts.
Ronaldo trails Lazio striker Ciro Immobile by four goals in the battle for Europe's Golden Shoe.
Immobile scored his 35th goal of the season as Lazio beat Brescia 2-0 on Wednesday.